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- DOI 10.18231/2394-6792.2018.0104
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CrossMark
- Citation
Evaluation of nucleic acid testing [NAT] of blood donors
- Author Details:
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Chaithanya K *
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Shivakumar S
Context: Human immunodeficiency virus (HIV), hepatitis C virus (HCV), and hepatitis B virus (HBV) are easily transmitted through infected blood, to prevent this serological screening of blood units is a routine norm. Many cases could be missed if the testing is done in window period. To overcome this and to provide an additional protection many tests with better sensitivity and specificity like Nucleic acid testing (NAT) have been tried.
Aims: To observe NAT reactivity trend for transfusion transmittable diseases, and to correlate ELISA and NAT methods in detecting HIV, HBV and HCV.
Materials and Methods: A six years retrospective observational study conducted in Blood bank, Department of Pathology. ELISA and ID-NAT results of the donor samples for HIV, HBV and HCV were collected in a specially designed proforma and analyzed.
Statistical Analysis used: Data entered in Microsoft excel sheets, means and percentages were calculated.
Results: In 6years 52,417 units of blood collected, 44,600(85.08%) were voluntary donors and 7,817(14.92%) replacement donors. There were 521(0.99%) cases of HBV, 98 (0.19%) HIV and 48 (0.09%) HCV and two cases of HIV-HBV co-infection. About 557 were positive by both ELISA and NAT method (1.06%). Fifty eight cases were positive only by ID-NAT method and negative on serological testing accounting to the NAT yield of 1 in 893 cases. There were 2 cases of HIV-2 which were missed by NAT and picked up in ELISA.
Conclusions: Screening of blood and blood products using dual testing with high sensitivity serological assays and NAT helps to detect potentially infectious diseases in all phases of infection.
Keywords: ID-NAT, ELISA, Transfusion transmittable diseases.
References
- NACO | National AIDS Control Organisation. Available rom :http://www.naco.gov.in/NACO/ National_AIDS_Control_Program/Services_for_Preventi on/ Access_to_Safe _blood/. [Last cited on 2018 Feb 06]. 2 Makroo RN, Hegde V, Chowdhry M, Bhatia A, Rosamma NL. Seroprevalence of infectious markers and their trends in blood donors in a hospital based blood bank in North India. Indian J Med Res. 2015;142:317‑22. 3 Voluntary blood donation programme -An operational Guideline, 2007. Available from: http://www.nacoonline.org/upload/Policies & Guidelines/29, voluntary blood donation.pdf 4 Hollinger FB, Liang TJ. Hepatitis B virus. In: Knipe DM et al. eds. Field virology, 4 th ed. Philadelphia, Lippincot Williams and Wilkins, 2001:2971-3036 5 Blood bank. Central Drugs Standard Control Organization. Guidelines for blood banks (updated 2012 Jul 26). Available from: www.cdsco.nic.in/forms/list, accessed on Jan 10, 2018. 6 Bhatia R Blood transfusion services in developing countries of South-East Asia. Transfus Today. 2005;65:5647-53. 7 Hans R, Marwaha N. Nucleic acid testing – benefits and constraints. Asian J Transfus Sci. 2014;8(1):2–3. 8 Coste J. Implementation of donor screening for infectious agents transmitted by blood by nucleic acid technology: Update to 200. Vox Sang. 2005;88:289-303. 9 Chatterjee K et al. Individual donor nucleic acid testing for blood safety against HIV-1 and Hepatitis B and C viruses in a tertiary care hospital. The National Medical Journal of India Vol 25, No.4,2012 10 Engelfriet CP, Reesink HW, Hernandez JM, Sauleda S, O′Riordan J, Pratt D, et al. Implementation of donors screening for infectious agents transmitted by blood by nuclic acid technology. Vox Sang. 2002;82:87-111. 11 Sultan S. Multiplex real-time RT-PCR assay for transfusion transmitted viruses in sero – negative allogenic blood donors: an experience from Southern Pakistan. Malaysian J Pathol. 2017;39(2):149–154 12 Taurifi HA. Evaluation of nucleic acid testing for blood donors: One year study. Int J Blood Transfus Immunohematol. 2015;5:19–25. 13 Kumar R, Gupta S, Kaur R, Gupta M. Individual donor – nucleic acid testing for human immunodeficiency virus – 1, hepatitis C virus and hepatitis B virus and its role in blood safety. Asian Journal of Transfusion Medicine. 2015;9(2):199-202. 14 Makroo R N. Multicenter evaluation of individual donor nucleic acid testing (NAT) for simultaneous detection of human deficiency virus – 1 & 2 hepatitis B &C viruses in Indian blood donors. Indian J Med Res. 127, February 2008, 140-147. 15 Naidu NK, Barucha ZS, Sonawane V, Ahmed I. Nucleic Acid Testing: Is it the only answer for safe blood in India?. Asian Journal of Transfusion Medicine. 2016;10(1):79-83. 16 Jain R, Aggarwal P, Gupta G N. Need for Nucleic Acid Testing in countries with high prevalence of Transfusion – Transmitted Infections. ISRN Hematology. 2012, Article ID 718671, 5 pages doi:10.5402/2012/718671
[Google Scholar] 17 Hans R, Marwaha N. Nucleic acid testing – benefits and constraints. Asian J Transfus Sci. 2014;8(1):2–3. 18 Pathak S, Chandrashekar M. Transfusion transmittable infections - Seroprevalence among blood donors in a tertiary care hospital of Delhi. Asian journal of transfusion medicine. 2013;7(2):116-118. 19 Agarwal N, Chatterjee K, Coshic P, Borgohain M. Nucleic acid testing for blood banks: An experience from a tertiary care centre in New Delhi, India. Transfusion and apheresis science. 2013;49(3):482-484. 20 Ghosh K, Mishra K. Nucleic acid amplification testing in Indian blood banks: A review with perspectives. Indian journal of Pathology and Microbiology. 2017;60(3):313- - 21 Makroo RN. Impact of routine individual blood donor nucleic acid testing (ID-NAT) for HIV-1, HCV and HBV on blood safety in a tertiary care hospital. Apollo Med. 2007;4:9-12. 22 Selim, H.M., ElBashaar, M.A. & ElWakil, S.G. Donor minipool NAT screening for HBV, HCV, and HIV: a 2- year experience in a private hospital in Saudi Arabia. Comp Clin Pathol. 2014;23:1125. 23 Stramer SL, Glynn SA, Kleinman SH, Strong DM, Caglioti S, Wright DJ, et al. Detection of HIV-1 and HCV infections among antibody-negative blood donors by nucleic acidamplification testing. N Engl J Med. 2004;351:760-8. 24 Hourfar MK, Jork C, Schottstedt V, Weber-Schehl M, Brixner V, Busch MP, et al. Experience of German Red Cross blood donor services with nucleic acid testing: Results of screening more than 30 million blood donations for human immunodeficiency virus-1, hepatitis C virus, and hepatitis B virus. Transfusion. 2008;48:1558-66. 25 Ohnuma H. The first large- scale nucleic acid amplification testing (NAT) of donated blood using Multiplex reagent for simultaneous detection of HBV, Chaithanya K et al. Evaluation of nucleic acid testing [NAT] of blood donors Indian Journal of Pathology and Oncology, October-December, 2018;5(4):536-541 541 HCV and HIV-1 and significance of NAT for HBV. Microbiol Immunol. 2001;45(9): 667-672. 26 B. Singh. “Nucleic Acid Testing (NAT) screening of blood donors in India,” A Project Report, International Hospital Federation Reference Book, 2008/2009. 27 M. Meena, T. Jindal, and A. Hazarika, “Prevalence of hepatitis B virus and hepatitis C virus among blood donors at a tertiary care hospital in India: a five-year study,” Transfusion. 2011;51(1):198–202.
How to Cite This Article
Vancouver
K C, S S. Evaluation of nucleic acid testing [NAT] of blood donors [Internet]. Indian J Pathol Oncol. 2018 [cited 2025 Oct 03];5(4):536-541. Available from: https://doi.org/10.18231/2394-6792.2018.0104
APA
K, C., S, S. (2018). Evaluation of nucleic acid testing [NAT] of blood donors. Indian J Pathol Oncol, 5(4), 536-541. https://doi.org/10.18231/2394-6792.2018.0104
MLA
K, Chaithanya, S, Shivakumar. "Evaluation of nucleic acid testing [NAT] of blood donors." Indian J Pathol Oncol, vol. 5, no. 4, 2018, pp. 536-541. https://doi.org/10.18231/2394-6792.2018.0104
Chicago
K, C., S, S.. "Evaluation of nucleic acid testing [NAT] of blood donors." Indian J Pathol Oncol 5, no. 4 (2018): 536-541. https://doi.org/10.18231/2394-6792.2018.0104