- Visibility 884 Views
- Downloads 788 Downloads
- Permissions
- DOI 10.18231/j.ijpo.2025.025
-
CrossMark
Efficacy of cytology in small round cell tumours
- Author Details:
-
Deepika Rani *
-
Nidhi Verma
Deepika Rani *
Background: Malignant small round cell tumours are characterized by small, round, relatively undifferentiated cells sharing similar histology when visualized under light microscope. These typically encompass Ewing’s sarcoma, peripheral neuroectodermal tumour (PNET), rhabdomyosarcoma, synovial sarcoma, non-Hodgkin’s lymphoma, retinoblastoma, neuroblastoma, hepatoblastoma, and nephroblastoma commonly known as Wilms’ tumour. Fine needle aspiration cytology (FNAC) has emerged as a vital diagnostic tool for these types of tumours.
Materials and Methods: This was a retrospective study of all diagnosed cases of pediatric small round cell tumours in Maulana Azad Medical College and Associated LNH Hospital, New Delhi between the period of 2016-2020. 30 cases of pediatric small round cell tumour were studied. Data was analysed using SPSS version 25 and MS Excel. Qualitative data was expressed as frequencies, percentages and proportions. Sensitivity and Specificity of FNAC in categorization of small round cell tumours was calculated.
Results: In 22 out of 30 cases, FNAC could correctly establish the correct nature of lesion with an overall diagnostic accuracy of 73.3%. Complete concordance (diagnosis of SRCT with further categorization) could be done in 22/30 (73.3%) cases, whereas discordance in subtyping was seen in 8/30 (26.6%) cases.
Conclusion: FNAC could correctly establish the correct nature of lesion with an overall diagnostic accuracy of 73.3%. Adequate clinical history and classical presentations assisted significantly in arriving at the final diagnosis. Clinical and radiological correlation aided with ancillary techniques increases the diagnostic accuracy of FNAC in Malignant small round cell tumour.
Keywords: Small round cell tumour, Fine needle aspiration cytology, Ewing’s sarcoma, rhabdomyosarcoma, non-Hodgkin’s lymphoma, Wilms’ tumour.
References
- Kim NR, Ha SY, Cho HY. Utility of Transmission Electron Microscopy in Small Round Cell Tumors. J Pathol Transl Med. 2015;49(2):93–101.
- Bahrami A, Truong LD, Ro JY. Undifferentiated tumour: true identity by immunohistochemistry. Arch Pathol Lab Med 2008;132(3):326–48.
- Halliday BE, Slagel DD, Elsheikh TE, Silverman JF. Diagnostic utility of mic-2 immunocytomchemical staining in the differential diagnosis of small blue cell tumors. Diagn Cytopathol. 1998;19(6):410–6.
- Collins BT, Cramer HM, Frain BE, Davis MM. Fine-needle aspiration biopsy of metastatic Ewing's sarcoma with MIC2 (CD99) immunocytochemistry. Diagn Cytopathol. 1998;19(5):382–4.
- Folpe AL, McKenney JK, Bridge JA, Weiss SW. Sclerosing rhabdomyosarcoma in adults: Report of four cases of a hyalinizing, matrix-rich variant of rhabdomyosarcoma that may be confused with osteosarcoma, chondrosarcoma, or angiosarcoma. Am J Surg Pathol. 2002;26(9):1175–83.
- Fisher C. Synovial sarcoma: Ultras tructural and immunohistochemical features of epithelial differentiation in monophasic and biphasic tumours. Hum Pathol. 1986;17(10):996–
- Silverman JF, Dabbs DJ, Ganick DJ, Holbrook CT, Geisinger KR. Fine needle aspiration cytology of neuroblastoma, including peripheral neuroectodermal tumor, with immunocytochemical and ultrastructural confirmation. Acta Cytol. 1988;32(3):367–76.
- McGahey BE, Moriarty AT, Nelson WA, Hull MT. Fine-needle aspiration biopsy of small round blue cell tumors of childhood. Cancer. 1992;69(4):1067–73. Rani and Verma / Indian Journal of Pathology and Oncology 2025;12(2):109–115 115
- Layfield LJ, Liu K, Dodge RK. Logistic regression analysis of small round cell neoplasms: a cytologic study. Diagn Cytopathol. 1999;20:271-7.
- Cohen MC, Pollano D, Tomarchio SA, Drut R. Cytologic characteristics of peripheral neuroectodermal tumors in fine needle aspiration smear: A retrospective study of three pediatric cases. Diagn Cytopathol. 1997;16(6):513–7.
- Pilotti S, Rilke F, Alasio L, Garbagnati F. The role of fine needle aspiration in the assessment of renal masses. Acta Cytol. 1988;32(1):1–10.
- Layfield LJ, Glasgow B, Ostrzega N, Reynolds CP. Fine-needle aspiration cytology and the diagnosis of neoplasms in the pediatric age group. Diagn Cytopathol. 1991;7(5):451–61.
- Maheshwari V, Alam K, Jain A, Aggarwal S, Chana RS. Diagnostic utility of fine needle aspiration cytology in pediatric tumors. J Cytol. 2008;25(2):45.
- Halliday BE, Slagel DD, Elsheikh TE, Silverman JF. Diagnostic utility of MIC‐2 immunocytochemical staining in the differential diagnosis of small blue cell tumors. Diagn Cytopathol. 1998;19(6):410–6.
- Leon ME, Hou JS, Galindo LM, Garcia FU. Fine‐needle aspiration of adult small‐round‐cell tumors studied with flow cytometry. Diagn Cytopathol. 2004;31(3):147–54.
- Gangopadhyay M, Chakrabarti I, Ghosh P, Ghosh N, Guha Mallick Sinha M. Fine needle aspiration cytology of non-hematological neoplasms in pediatric age group: Our experience. J Cytol. 2015;32(3):165–9.
- Asim M, Mudassir G, Hashmi AA, Abid M, Sheikh AK, Naveed H, et al. Diagnostic accuracy of fine needle aspiration biopsy in pediatric small round cell tumors. BMC Res Notes. 2018;11:573.