Efficacy of cell block immunocytochemistry in the diagnostic workup of non hodgkin lymphoma-experience from a tertiary care hospital in North India

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Original Article

Author Details : Pradyumn Singh, Anurag Gupta, Namrata P Awasthi, Ashish Singhal, Gaurav Gupta

Volume : 5, Issue : 2, Year : 2018

Article Page : 216-220

https://doi.org/10.18231/2394-6792.2018.0041

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Abstract

The WHO 2016 update on lymphoid neoplasms incorporates criteria independent of tissue architecture in the diagnosis and sub-classification of non-Hodgkin lymphoma. Thus, lymphoid neoplasms with characteristic immunophenotypes and/or cytomorphology could be classified with FNA material with appropriate use of ancillary methods. We analysed the efficacy of cytomorphology and cell block immunocytochemistry (CB-ICC) in in diagnostic workup of non-Hodgkin lymphoma diagnosis on FNA material. 65 clinically suspected cases of lymphoma presenting with lymphnode enlargements were analysed. FNAC and CB-ICC were performed and compared with the subsequent biopsy. The sensitivity, specificity, PPV and NPV of CBICC in the diagnosis of NHL were 84.6%, 75%, 95.6%, and 42.8%, respectively in our study.
We also observed that use of CB-ICC can have significant logistic implications in patient management in terms of reducing the turn-around time to diagnosis and improving the accuracy in diagnosis on FNAC. ICC on cell blocks can easily be done on FNA material and is a very useful adjunct to FNAC in establishing the diagnosis in a suspected case of NHL especially when a biopsy is not available.

Keywords: Cell block immunocytochemistry, NHL, FNAC, Ancillary methods.

How to cite : Singh P, Gupta A, Awasthi N P, Singhal A, Gupta G, Efficacy of cell block immunocytochemistry in the diagnostic workup of non hodgkin lymphoma-experience from a tertiary care hospital in North India. Indian J Pathol Oncol 2018;5(2):216-220

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