Comparative Immunohistochemical Analysis of Ki -67 in a Spectrum of Pediatric Solid Tumours

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Original Article

Author Details : GA Vasugi, Sandhya Sundaram, Lawrence D’cruze, Aruna Rajendran, Julius Xavier Scott

Volume : 4, Issue : 4, Year : 2017

Article Page : 570-574

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Abstract

Introduction: The aim of this study is to analyse the clinicopathological profile of malignant pediatric solid tumours and also to evaluate the proliferative index using Ki-67 in a spectrum of these tumours.
Materials and Method: 75 cases of malignant solid tumours age group less than 17 years, reported at Sri Ramachandra Medical College from Jan 2009 to Dec 2013 are included in this study. Formalin fixed paraffin embedded tissue stained with H&E were used for routine morphology. Immunohistochemical staining was done using monoclonal anti Ki 67 antibody by Streptavidin biotin peroxidase complex technique. Labelling index or growth fraction is calculated as the percentage of positive cells for 1000 cells evaluated.
Results: Among the 75 cases 52 (68%) were males and 23(32%) were females. 22(29%) were CNS neoplasms and 53(71%) were Non CNS neoplasms which included lymphoma, Ewing’s sarcoma, Osteosarcoma, Wilms tumour, Soft tissue tumours, Germ cell tumours, Neuroblastoma, Hepatoblastoma & others. The labelling index was variable according to the grade of the tumours. It varied from 2 to 40% in CNS neoplasms according to the grade and about 1 to 90% in various Non CNS neoplasms.
Conclusion: Pediatric solid tumours form a wide spectrum. Ki-67 labelling index may be a useful adjunct in predicting the biological behaviour & prognosis of pediatric solid tumours.

Keywords: Ki-67, Pediatric solid tumours, prognosis

How to cite : Vasugi G, Sundaram S, D’cruze L, Rajendran A, Scott J X, Comparative Immunohistochemical Analysis of Ki -67 in a Spectrum of Pediatric Solid Tumours. Indian J Pathol Oncol 2017;4(4):570-574

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