A comparative study of red cell histogram along with CBC parameters and peripheral blood smear in various anemias

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Original Article

Author Details : Durgesh Kumar Dhakar*, Naresh N Rai

Volume : 10, Issue : 2, Year : 2023

Article Page : 156-162

https://doi.org/10.18231/j.ijpo.2023.032

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Abstract

Introduction: The red blood cell (RBC) parameters generated by cell counters can aid in morphological categorization of anemia, the significance of microscopic analysis of peripheral blood smears (PBSs) cannot be overlooked when it comes to interpreting the underlying cause of anemia. Therefore, both RBC parameters and microscopic examination of PBSs are essential for a comprehensive assessment of anemia.
Aims and Objective: This study aimed to evaluate and compare the results obtained through two different methods: examination of peripheral blood smears (PBS) and automated cell counter generated parameters. The objective was to identify any differences or similarities in the diagnostic outcomes produced by each method.
Materials and Methods: Over the course of one year, from December 2020 to November 2021, a hospital-based, prospective study was conducted. The study aimed to evaluate and compare the PBS findings and RBC parameters and histograms generated by a cell counter in 1000 anemic patients.
In a sample of 1000 cases, we compared the typing of anemia using an automated analyzer and peripheral smear examination. Out of these cases, 690 showed consistent typing results between the two methods, while 310 showed discordant typing. The automated analyzer diagnosed majority of cases 70.1% as microcytic anemia and Normocytic, dimorphic, and macrocytic cases were found in 13.5%, 7.8%, and 4.3% of cases, respectively. Additionally, a normal histogram was observed in 17.6% of cases.
Conclusion: We have concluded that while the automated analyzer is useful in reducing the overall workload through its graphical representation of anemia typing, it should always be confirmed through microscopy. The automated analyzer is a helpful tool, it should not be relied upon solely for accurate anemia typing. Microscopy remains an essential method for confirming the results of automated analysis.
 

Keywords: Anemia, Peripheral blood smear, Automated cell counter, Red blood cell.

How to cite : Dhakar D K, Rai N N, A comparative study of red cell histogram along with CBC parameters and peripheral blood smear in various anemias. Indian J Pathol Oncol 2023;10(2):156-162

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